SPC365

Genetic polymorphisms associated with the onset of Essential Hypertension in the Diabetic population

Session: Posters - I. Hypertension
Speaker: Ana Célia Sousa

Congress: CPC 2021
Topic: I. Hypertension
Theme: 27. Hypertension
Subtheme: 27.1 Hypertension – Pathophysiology and Mechanisms
Session Type: Posters
FP Number: ---
Authors: Ana Célia Sousa; Mariana Bilreiro; Carolina Aguiar; Bela Machado; Eva Henriques; Sónia Freitas; Mariana Rodrigues; Sofia Borges; Ana Isabel Freitas; Graça Guerra; Ilídio Ornelas; Roberto Palma Dos Reis; Maria Isabel Mendonça

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;">Essential </span></span><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;">hypertension (EH) is a common pathology in patients with Diabetes Mellitus, which implies a substantial increase in cardiovascular risk and can awaken or accelerate micro and macrovascular lesions of diabetes. The development of EH in diabetics can be genetically determined, however it is unknown which genetic polymorphisms may be involved in the onset of EH in diabetics.</span></span></span></span></p>

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;">Objective:</span></span></strong><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;"> Investigate the genetic factors associated with the onset of EH in diabetic patients.</span></span></span></span></p>

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;">Methods:</span></span></strong><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;"> With a sample of 203 diabetic individuals, two groups were constituted, according to whether they had (n=163) or not (n=40) EH. The following genetic variants were analyzed: ACE I/D rs4340, ACE A2350G rs4343, AGT T174M rs4762, AGT M235T rs699, AGTR1 A1166C rs5186, CYP11B2 -344 C/T rs1799998, ADD1 G460W rs4961, SCNN1G G-173A rs5718, ADRB1 R389G rs1801253, ADRB2 R16G rs1042713, CYP17A1 T/C rs11191548, GNB3 C825T rs5443, ATP2B1 A/G rs2681472, in order to determine which genotypic frequencies are associated with EH in diabetics and calculated 4 genetic models (dominant, recessive, additive and multiplicative. Finally, a multivariate analysis was performed to determine which genetic variants are significantly and independently associated with EH in diabetics.</span></span></span></span></p>

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;">Results:</span></span></strong><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;"> The group of diabetic patients with EH was composed of 62% male (mean age 54.8 &plusmn; 6.9 years) and the control group without EH comprised 67.5% male (mean age 54.2 &plusmn; 6.5 years). Of the 13 studied genetic variants, AGTR1 was associated with the onset of EH in 3 models: dominant (OR 3.754; p=0.001), additive (OR 3.095; p=0.002) and multiplicative (OR 3.097; p=0.001). An association was also found in ADD1 in the multiplicative model (OR 2.449; p=0.041) and GNB3 in the dominant model (OR 2.135; p=0.032). After multivariate analysis, CT+TT genotypes of GNB3 and AC+CC of AGTR1 were independent predictors of EH in the diabetic population with an OR of 2.360 (p=0.021) and OR of 4.010 (p=0.001), respectively.</span></span></span></span></p>

<p style="text-align:justify"><span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;">Conclusion:</span></span></strong><span style="font-size:12.0pt"><span style="font-family:&quot;Arial&quot;,&quot;sans-serif&quot;"> The genetic variants GNB3 and AGTR1 were significantly and independently associated with the EH onset in the diabetic population. This result points to the existence of polymorphic genetic alterations that favor the onset of EH in the diabetic population. Diabetic individuals who have these genetic variants, with greater predisposition to EH onset, should have special careful in behavioral terms, such as salt consumption, to counteract this genetic trend and, thus, reduce cardiovascular risk.</span></span></span></span></p>